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--- highlights:2025:membranespxct [2026/02/01 13:11] – Ronell Sicat
+++ highlights:2025:membranespxct [2026/02/02 13:51] (current) – Ronell Sicat
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-This project investigates the interactions of microrganisms with carbonate grains and corals-derived sediment in shallow-marine environments and how
+This collaboration between KAUST and the Brazilian Center for Research in Energy and Materials (CNPEM) resulted in the development of a high-resolution 3D imaging methodology that allows 3D nanoscale imaging of soft matter in situ. Unlike traditional techniques that require vacuum or sample modifications – which can distort structures and sample physicochemical properties - this method captures the structure directly in water (without vitrification/freezing). The methodology was used to capture the structure of thin film composite polyamide membrane (typically used for water purification) directly in its hydrated state – close to operational conditions. The sample did not have to be modified with typical heavy-metal stains or separated from the polysulfone, which allows preservation of the membrane’s native state. Using the developed approach led to observations of substantial swelling and structural changes of the polyamide membrane with artificial water channels, providing new insights that can guide the design of more efficient water purification materials. This novel method and observations based on in situ ptychographic x-ray computed tomography using a synchrotron source are presented in the recently published article in Langmuir (details below). The method has a huge potential to be applied to the study of soft matter in hydrated environments in its native state, ranging from polymers to biological samples.
-these interactions alter rocks and sediments over time. By combining in situ field experiments with X-ray micro–computed tomography (μCT) and
-Backscatering electron microscopy (BS-SEM), researchers were able to track these transformations in three dimensions and through time, directly visualizing microbial microboring, micrite formation, and the development of microporosity at the grain scale. This mutidisciplinary approach helps
+**In Situ Ptychographic X-ray Computed Tomography of Fully Hydrated Polyamide Membranes.** Radosław Górecki, Ronell Sicat, Carla Cristina Polo, Tiago Araujo Kalile, Maria Di Vincenzo, Florian Meneau, and Suzana P. Nunes. Langmuir Article. [[https://pubs.acs.org/doi/10.1021/acs.langmuir.5c04933|DOI: 10.1021/acs.langmuir.5c04933]].
-clarify the role of microbes in controlling early alteration processes in carbonate sediment/rock and how these processes shape rock properties of
-carbonates.
-{{:wiki:highlights:2025:teillet.jpeg?1000|}}
+{{:wiki:highlights:2025:pxct1.png?800|}}
-Figure from [[https://pubs.geoscienceworld.org/sepm/books/edited-volume/2828/chapter/153154491/Micritized-Grains-and-Peloids-The-Taphonomic|preliminary report]] showing A,B,C: experiment sites, D: carbonate samples, E,F: thin sections of carbonates.
+This figure from the paper shows the whole workflow including ptychographic x-ray computed tomography (PXCT), reconstruction, segmentation, and analysis.
 \\
-Preliminary results of this project were presented by the lead researcher Thomas Teillet (former KAUST postdoc) at the [[https://www.imageevent.org/|International Meeting for Applied Geoscience and Energy (IMAGE) Conference]] as well as at the [[https://www.sepm.org/mountjoy-2025|Mountjoy Carbonate Conference]], both in August 2025. More information can also be found in a preliminary report in GeoScienceWorld entitled: [[https://pubs.geoscienceworld.org/sepm/books/edited-volume/2828/chapter/153154491/Micritized-Grains-and-Peloids-The-Taphonomic|"Micritized Grains and Peloids: The Taphonomic Adventures of Carbonate Grains in Shallow Marine Settings"]].
+KVL's visualization scientist Ronell Sicat provided support in setting up the ptychographic and tomographic reconstruction workflows in the [[https://docs.hpc.kaust.edu.sa/systems/ibex/index.html|Ibex cluster]] (maintained by the KAUST Supercomputing Core Lab). He also worked closely with the domain experts in pre-processing, segmentation, analysis, and visualization of the reconstructed 3D image stacks using Avizo software.
-KVL's visualization scientist Dr. Ronell Sicat supported Dr. Teillet in processing the μCT data using Avizo software, including preprocessing, alignment, segmentation, and analysis, in order to quantify changes in the rock samples over time (see below image).  A full paper is currently underway with a more detailed analysis of the microbial interactions with the rock samples.
-{{:wiki:highlights:2025:carbonates.png?1000|}}
-Example Avizo segmentation and analysis results for one of the rock samples.
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